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Mitochondrial Morphology of MEF and HEK mito-YFP and mito Ds-Red cells. (A) Immunoblot analysis of the expression level of mitochondrial fusion machinery (OPA1, MFN1 and MFN2) as well as Flag-HRasG12V in a panel of separately labeled fluorescent sets (mito-YFP or mito-DsRed) of MEF and HEK cells. GAPDH, loading control. (B–F) Mitochondrial morphologies of the panel of cells examined by immunoblot in (A). Green, mito-YFP; Red, mito-DsRed; Blue, DAPI. Scale bar, 20 μm.
