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. Author manuscript; available in PMC: 2017 Jul 10.
Published in final edited form as: Cytometry A. 2016 Jul 7;89(8):708–719. doi: 10.1002/cyto.a.22891

Figure 3.

Figure 3

Gating Strategy for identification of double positive Mito-YFP/Mito-DsRed fused cells in the IDEAS Software. (A) Gating of focused cells according to the gradient RMS feature above 30 arbitrary units. (B) The single cells were gated based on their aspect ratio and area in the brightfield channel. (C) Gating of High DAPI expressing cells was based on high DAPI positivity according to DAPI fluorescence intensity. (D) Gating of the double positive mito-YFP/mito-DsRed expressing cells according to fluorescence intensity of YFP and DsRed.