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. Author manuscript; available in PMC: 2017 Jul 10.
Published in final edited form as: Cytometry A. 2016 Jul 7;89(8):708–719. doi: 10.1002/cyto.a.22891

Figure 4.

Figure 4

Establishment of co-localized and non-co-localized cutoffs based on Positive and Negative Mitochondrial Fusion Controls. (A–B) Representative images of HEK-TtH co-expressing Mito-YFP and Mito-DsRed as the positive fusion control (A) or MFN DKO MEFs after PEG treatment as the negative fusion control(B). White number, Cell number; Yellow number, co-localization score. (C–D) Single merged YFP/DsRed Image of positive control (C) or negative control (D). (E–F) Histograms depicting the range of co-localization scores and statistics for the positive control (E) and negative control (F).