Table 2.
Mutagenicity of rice bran extracts using the Salmonella mutation assay in TA98 and TA100, with or without metabolic activation
| Treatment | Concentration (per plate) | Number of His+ revertant colonies | |||||||
|---|---|---|---|---|---|---|---|---|---|
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| |||||||||
| TA98 (+S9) | TA98 (−S9) | TA100 (+S9) | TA100 (−S9) | ||||||
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| DCM | MeOH | DCM | MeOH | DCM | MeOH | DCM | MeOH | ||
| DMSO | 50 μL | 30.1 ± 2.1 | 24.7 ± 0.6 | 117.7 ± 5.3 | 123.8 ± 4.4 | ||||
| AF-2 | 0.01 μg | - | - | - | 509.1 ± 19.4 | ||||
| AF-2 | 0.1 μg | - | 347.0 ± 16.9 | - | - | ||||
| 2-AA | 0.5 mg | 416.1 ± 21.1 | - | 612.0 ± 27.3 | - | ||||
| Purple rice bran | 0.2 mg | 30.7 ± 2.4 | 33.0 ± 3.2 | 20.1 ± 1.2 | 22.6 ± 0.6 | 105.4 ± 3.1 | 122.9 ± 3.1 | 133.1 ± 6.5 | 126.4 ± 3.7 |
| 1.0 mg | 28.6 ± 2.5 | 35.3 ± 2.8 | 18.9 ± 0.6 | 22.8 ± 1.2 | 107.9 ± 5.7 | 116.6 ± 6.3 | 139.1 ± 3.3 | 126.9 ± 2.9 | |
| 5.0 mg | 25.8 ± 2.5 | 41.0 ± 3.1 | 17.9 ± 0.5 | 24.8 ± 2.3 | 111.8 ± 6.7 | 158.9 ± 8.0 | 124.4 ± 5.2 | 113.4 ± 7.6 | |
| White rice bran | 0.2 mg | 32.1 ± 4.0 | 31.2 ± 3.0 | 19.3 ± 0.7 | 23.8 ± 1.0 | 104.4 ± 5.6 | 117.3 ± 3.2 | 112.4 ± 5.6 | 119.6 ± 6.7 |
| 1.0 mg | 26.1 ± 3.5 | 30.4 ± 2.2 | 18.9 ± 0.5 | 21.3 ± 0.9 | 98.4 ± 5.4 | 121.7 ± 5.6 | 91.6 ± 2.9 | 106.3 ± 5.0 | |
| 5.0 mg | 28.8 ± 3.3 | 32.6 ± 2.6 | 18.1 ± 0.5 | 22.2 ± 0.9 | 109.4 ± 6.0 | 132.7 ± 4.4 | 93.7 ± 3.0 | 85.0 ± 1.9 | |
Values are presented as mean ± SEM. S9, S9 mix; DCM, dichloromethane extract; MeOH, methanol extract; DMSO, dimethyl sulfoxide; AF2, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide; 2-AA, 2-aminoanthracene.