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. 2017 Jul 10;12(7):e0180163. doi: 10.1371/journal.pone.0180163

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© 2017 Abeyrathne, Grigorieff

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — Peak 1 corresponds to the void volume. Peak 2 corresponds to ClC-rm1_eGFP. (b) SDS-PAGE gel of ClC-rm1_eGFP after SEC. Lane 1: molecular mass marker. Lane 2 ClC-rm1_eGFP after SEC. ClC-rm1 monomer band is around ~80 kDa. (c) Micrograph of negatively stained ClC-rm1_eGFP particles. DDM-solubilized ClC-rm1_eGFP particles are mono-disperse. Scale bar 20 nm (d) Representative class averages of ClC-rm1_eGFP in DDM.