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. 2017 Jul 10;12(7):e0180650. doi: 10.1371/journal.pone.0180650

Fig 4. Effect of 5-FU and AA on cell survival in the dentate gyrus.

Fig 4

Representative images of BrdU positive cells in the dentate gyrus in each group (A-F). BrdU positive cells were stained green in the subgranular zone (SGZ) of the dentate gyrus. Sections were counterstained with red nuclear dye, propidium iodide: PI. Arrowheads indicate BrdU positive cells in the the dentate gyrus (scale bars: 100 μm). Inserted images show BrdU immunostaining under high magnification (scale bar: 50 μm). Mean BrdU positive cell counts in the 5-FU group were significantly lower than the control, AA, and 5-FU+AA (preventive and throughout) groups (*p<0.05, G). Furthermore, BrdU positive cell counts in the 5-FU+AA (recovery) group were significantly less than those in the control group (#p<0.05, G). ML: molecular layer, GCL: granule cell layer.