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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Regen Eng Transl Med. 2017 May 8;3(2):82–93. doi: 10.1007/s40883-017-0030-2

Figure 3. Monocyte subpopulations.

Figure 3

(A) Flow cytometry gating scheme to identify Ly6Clo and Ly6Chi subpopulations of monocytes. The fraction of Ly6Clo (B) and Ly6Chi (C) cells within the monocyte pool are increased and decreased, respectively, by TT+DN injury. The numbers of Ly6Clo and Ly6Chi monocytes per gram of muscle are increased by injury (D). Closed circles represent the injured side; open circles, uninjured contralateral side. Statistical comparisons are conducted by repeated measures two-way ANOVA followed by Sidak multiple comparisons test (TT, n=5; TT+DN, n=4). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 comparing injury side to contralateral side. ^P<0.05, ^^P<0.01, ^^^P<0.001 comparing TT+DN to TT.