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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 May 26;199(1):336–347. doi: 10.4049/jimmunol.1502181

Figure 1. Blockade of CD70 exacerbates GVHD.

Figure 1

(A) BALB/c and C57BL/6 mice were given 792 and 965 cGy irradiation, respectively, on day -1 and transplanted day 0 with 2×106 WT C57BL/6 BM only (n=2-4) ± 3×106 C57BL/6 splenocytes (spl) (n=4-10). Recipient mice were either treated with 25μg control IgG or 25μg anti-CD70 blocking antibody 4 and 6 days post-transplant and then monitored for survival. Results are pooled from two individual experiments. (B-C) BALB/c mice were given 792 cGy irradiation on day -1 and transplanted day 0 with 2×106 WT BM (n=4) ± 3.5×106 WT splenocytes (n=7) or 2×106 CD70-/- BM (n=4) ± 3.5×106 CD70-/- splenocytes (n=8). Mice were then monitored for weight loss (B) and survival (C). (D-E) BALB/c mice were given 792 cGy irradiation on day -1 and transplanted with 2×106 WT BM (n=5) ± 0.4×106 WT or CD70-/- CD25- PanT (n=5). Mice were then monitored for weight loss (D) and survival (E). In (A, C, E) data are presented as percent survival. In (B, D) data are presented as mean ± SEM. Statistical significance evaluated by log rank (Mantel-Cox) test. *P<0.05.