Figure 6. Residual host lymphocyte number and anti-donor function are not affected by CD70.

(A) WT or CD70^-/- C57BL/6 mice were transplanted and spleen cells were analyzed as described in Fig. 5 legend on days 3, 4, 5 and 7 after allo-HCT. Absolute numbers of host-derived CD4⁺ and CD8⁺ T cells in WT and CD70^-/- hosts were acquired via the following equation: (total number of splenocytes) × (percentage of live H-2K^b+CD3⁺CD4⁺ or H-2K^b+CD3⁺CD8⁺ T cells). Summary data from three individual experiments are shown (n=2-12 for each time point). Host T cells (A) and NK cells (B) in non-irradiated WT or CD70^-/- C57BL/6 mice were also provided. (C) One dose of 200μg NK1.1 antibody (PK136) or IgG control was injected on day -2. The host mice then received 965 cGy irradiation on day -1 and were subsequently transplanted on day 0. Survival data of hosts receiving 4×10⁶ BALB/c BM ± 3×10⁶ PanT are pooled from two individual experiments (n=8-14 for each group). Statistical significance evaluated by log rank (Mantel-Cox) test. *P<0.05; ***P<.001.