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. 2017 Jul 4;12:4773–4788. doi: 10.2147/IJN.S134378

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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Transfection of nanoparticles in vitro.

Notes: (A) Fluorescent micrographs of transfection of DDN at different ratios. (B and C) Flow cytometric histogram profiles of fluorescence intensity of DDN in HepG2 cells and B16 cells, respectively. (D) Transfection efficiency of DDN in the two kinds of cells following 48 h of incubation, with PEI as control. (E) Transfection efficiency of HDDN in the presence or absence of 10% FBS following 48 h of incubation.

Note: *P<0.05.

Abbreviations: DDN, doxorubicin-4-hydrazinobenzoic acid-polyethyleneimine conjugate/DNA nanoparticles; FBS, fetal bovine serum; HDDN, hyaluronic acid-shielded pH and enzyme dual-responsive nanoparticles; PEI, polyethyleneimine.