Figure 6. TRIP-Br1 binds to multiple AC isoforms and reduces their protein level and cAMP production.
(a) GST-TRIP-Br1 captured ACs 2, 4, 5, 6, and 8. (b) Stable expression of TRIP-Br1-V5 reduced the protein expression of ACs 1, 2, 4, 5, 6, and 8 that were transiently expressed in HEK293T cells. (c–d) Quantification of protein levels of various AC isoforms in panel b and their forskolin-stimulated cAMP production. cAMP production is normalized relative to that of the control of mock cells. *, different from control, p≤0.048; **p≤0.009; ***p=0.001; n = 3–5. (e) Sequence alignment of the AC isoforms used in panel b to identify conserved Lys residues (boxed). Yellow highlight: identical residues; blue highlight: highly conserved.