Appendix 2—table 2.

Summary of the assigned intermolecular-NOE between the ISS-N1-u25g RNA and the UP1-R140A protein variant. These NOEs were observed in the 2D ¹³C F2-filtered (¹H,¹H)-NOESY or the 3D ¹³C F1-edited, F3-filtered [¹H; ¹³C; ¹H] NOESY-HSQC or in both spectra. The intensity of the reported intermolecular-NOE peaks are classified regarding their signal/noise ratio as follows: - weak: 7 ≤ S/N <12 - middle: 12 ≤ S/N <25 - strong: 25 ≤ S/N <45 - very strong: 45 ≤ S/N.

DOI: http://dx.doi.org/10.7554/eLife.25736.032

Nucleotide	Atom	Protein residue and atom	Intensity of inter-NOE
CYT11	H1´	F108QE F108HZ F148QD	weak weak middle
	H5	E176HB2 E176HB3 E176QG R178QD	middle middle strong middle
ADE12	H1´	F108QE M137QE F148QD F148QE F148HZ F150QD F150QE A180QB L181QD1 L181QD2 K183QB K183QG K183QE	weak middle middle middle middle middle middle middle weak weak weak weak middle
	H2	R178QD A180QB L181QD1 L181QD2	strong strong strong very strong
	H8	F108QE E176HB2 E176HB3 E176QG R178QD A180QB L181QD1 L181QD2	weak middle middle strong strong strong middle very strong
GUA13	H1´	M137QE F148QE F150QE	very strong weak weak
	H8	K106QE M137HG2 M137QE F148QE F150QE K183QE	weak middle strong weak weak middle
ADE22	H1´	F17QE G19HA3 F57HB2 F57HB3 F57QD	very strong weak weak weak strong
	H8	F17QE	weak
ADE23	H1´	F17QE F17HZ M46QE F57HZ F59QE F59HZ	middle middle middle middle middle middle
	H2	F17HZ M46QE F59HB2 F59HB3 F59QD F59QE A89HA A89QB V90HB	strong middle middle middle strong strong middle very strong middle
	H8	F17QD F17QE	middle middle
GUA24	H1´	M46QE F59QE	very strong weak
	H2´	M46QE F59QE F59HZ	very strong middle middle
	H3´	M46QE F57HZ F59QD F59HZ	very strong middle middle middle
	H4´	V44QG1 M46QE	middle very strong
	H8	M46QE F57QE F59QD F59QE F59HZ	very strong middle middle middle middle
GUA25	H1´	V44HB V44QG1 V44QG2 M46QE F59QD F59HZ	middle very strong very strong very strong weak middle
	H8	M46QE	very strong