Table 3.
Evaluation of RNA specificity and affinity.
DOI: http://dx.doi.org/10.7554/eLife.25736.010
Table 3—source data 1. ITC data for evaluation of RNA specificity and affinity of the RRMs of hnRNP A1.
elife-25736-table3-data1.xlsx (47.6KB, xlsx)
DOI: 10.7554/eLife.25736.011
| RRM1 WT | RRM1 F23A | RRM1 D42A | RRM1 R92A | |
|---|---|---|---|---|
| UUAGGUC | 288 ± 16 (0.99 ± 0.05) | 225 ± 13 (1.07 ± 0.05) | 787 ± 42 (0.93 ± 0.05) | 1000–5000 (1.08 ± 0.05) |
| UAGGUC | 625 ± 35 (0.96 ± 0.05) | 602 ± 34 (0.91 ± 0.05) | ||
| UUUUAGGUC | 159 ± 9 (0.90 ± 0.05) | 130 ± 8 (0.91 ± 0.05) | ||
| UCAGGUC | 543 ± 33 (0.90 ± 0.05) | |||
| UUAGUU | 3000–9000 (0.97 ± 0.05) | 1000–5000 (1.07 ± 0.05) | >10’000 (1) | |
| UCAGUU | >10’000 (1) | |||
| UAAGUU | 3000–9000 (0.97 ± 0.05) | |||
| UGAGUU | 3000–9000 (0.99 ± 0.05) |
| RRM2 WT | RRM2 R140A | RRM2 E176Q | RRM2 Y167F | RRM2 M186A | |
|---|---|---|---|---|---|
| UCAGUU | 541 ± 33 (1.04 ± 0.05) | 1000–5’000 (1.07 ± 0.05) | 237 ± 15 (1.01 ± 0.05) | 513 ± 30 (0.99 ± 0.05) | 1630 ± 45 (0.96 ± 0.05) |
| UUAGUU | 125 ± 9 (1.04 ± 0.05) | 433 ± 26 (1.10 ± 0.05) | 286 ± 12 (0.99 ± 0.05) | 118 ± 9 (0.95 ± 0.05) | 253 ± 15 (1.00 ± 0.05) |
| UUAGGUC | 64 ± 8 (1.09 ± 0.05) | 195 ± 12 (1.01 ± 0.05) | 116 ± 39 (1.01 ± 0.05) | ||
| UCAGGUC | 129 ± 11 (1.01 ± 0.05) | 862 ± 47 (0.92 ± 0.05) | 132 ± 29 (0.93 ± 0.05) | ||
| UAAGUU | 543 ± 31 (1.04 ± 0.05) | 1000–5’000 (0.97 ± 0.05) | 429 ± 27 (0.94 ± 0.05) | ||
| UGAGUU | 546 ± 31 (0.93 ± 0.05) | 1000–5’000 (1) | 324 ± 20 (1.01 ± 0.05) | ||
| UAGGUC | 98 ± 8 (1.01 ± 0.05) | 685 ± 40 (1.01 ± 0.05) | 227 ± 14 (1.07 ± 0.05) |
Dissociation constant (Kd) for RRM1 and RRM2 wild-type and mutants, respectively. Values are reported as means ± standard error (S.E.). The uncertainties on the fitted parameters were estimated from the data spread and from the uncertainties of the protein concentration determination (5%). A dissociation constant range instead of a value is given where no sufficient saturation could be reached for the measurement conditions. The dissociation constants are given in nM. The number of sites N is given in parenthesis.