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. 2017 Apr 8;8(24):38084–38098. doi: 10.18632/oncotarget.16970

Figure 3. BEZ235 inhibits phosphorylation of AKT targets in human retinoblastoma cell lines.

Figure 3

A. WERI-Rb1 and B. Y79 cell lines were grown in media containing 0.25% serum, in 0.25% serum before stimulating with 20% serum for an hour, or grown continuously in 20% serum. Vehicle or BEZ235 (50 μM) was added one hour prior to harvesting cells for protein isolation. Proteins extracts were analyzed by immunoblotting for levels of p-FOXO1 (Ser256), FOXO1, p-AKT (Ser473), AKT, p-S6 (Ser235/236), S6, and ACTIN. Band densities were quantified using ImageJ and reported under each band. Numbers are only comparable to each other within the same gel.