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. 2017 Apr 27;8(24):38113–38135. doi: 10.18632/oncotarget.17494

Figure 7. GATA3 binds to NOX1 promoter region -321/−315.

Figure 7

A. Schematic illustration of the NOX1 promoter region and its variants generated by mutagenesis, highlighting the potential GATA3 binding sites. Mutated binding sites are shown in red. These promoter regions were individually constructed as PGL3 reporters. B. Mutation at the -321/−315 region abolishes luciferase activity associated with IL-4-mediated NOX1 promoter activity. HT-29 cells were transfected with the individual reporter constructs in the presence or absence of IL-4 (50 ng/ml), and luciferase activity was measured 48 h later. Data represent the mean ± SD of three experiments. *** = P < 0.001; n.s. = not significant.