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. 2017 Apr 7;8(24):38193–38213. doi: 10.18632/oncotarget.16942

Figure 4. Oxygen and nutrient availability differentially affect colorectal cancer cell survival and proliferation.

Figure 4

A. HCT-116 and HT-29 cells were incubated in progressively lower oxygen concentrations in the absence or presence of HGF, and apoptosis was determined by the annexin/propidium iodide method. The percentage of cells in Sub-G1 (inset) was determined by DNA content analysis (see Suppl. Figure S3 for full cell cycle distribution data). B. HCT-116 and HT-29 cells were incubated in progressively lower glucose concentrations in the absence or presence of HGF. Apoptosis and cell cycle distribution was determined as in A. Values represent mean ± SEM. Statistical significance was calculated using a Student's T-test (n = 3).