Table 1. KRAS/BRAF-mutant CRC cells rely on glucose more strictly than they depend on oxygen for survival.
| % apoptosis at 0.1% oxygen |
% apoptosis at 0.1 g/L glucose |
|||||
|---|---|---|---|---|---|---|
| CELL LINE | KRAS | BRAF | CTR | HGF | CTR | HGF |
| CACO-2 | WT | WT | 3.7 ± 0.1 | 2.5 ± 0.2 | 7.3 ± 0.4 | 4.2 ± 0.1 |
| COLO-320-DM | WT | WT | 3.3 ± 0.2 | 2.4 ± 0.2 | 6.5 ± 0.4 | 3.3 ± 0.2 |
| LIM-1215 | WT | WT | 3.6 ± 0.3 | 2.4 ± 0.2 | 5.7 ± 0.4 | 4.1 ± 0.2 |
| SW-48 | WT | WT | 4.6 ± 0.4 | 3.6 ± 0.2 | 6.5 ± 0.4 | 3.9 ± 0.2 |
| DLD-1 | G13D | WT | 5.7 ± 0.4 | 4.3 ± 0.2 | 38.4 ± 1.3 | 20.6 ± 0.8 |
| HCT-116 | G13D | WT | 6.7 ± 1.2 | 4.5 ± 0.9 | 40.4 ± 1.8 | 21.4 ± 1.8 |
| LOVO | G13D | WT | 6.0 ± 0.4 | 4.4 ± 0.4 | 33.6 ± 1.0 | 17.1 ± 0.7 |
| LS-1034 | A146T | WT | 5.0 ± 0.4 | 3.0 ± 0.2 | 25.5 ± 1.7 | 12.5 ± 0.6 |
| LS-123 | G12S | WT | 5.2 ± 0.6 | 2.8 ± 0.3 | 20.1 ± 1.0 | 11.0 ± 0.9 |
| LS-174T | G12D | WT | 6.1 ± 0.4 | 4.3 ± 0.4 | 32.4 ± 1.2 | 20.5 ± 0.6 |
| LS-180 | G12D | WT | 6.6 ± 0.4 | 4.5 ± 0.3 | 26.1 ± 1.2 | 14.0 ± 0.7 |
| SW-480 | G12V | WT | 5.9 ± 0.2 | 4.0 ± 0.1 | 24.0 ± 0.9 | 12.3 ± 0.9 |
| HT-29 | WT | V600E | 7.5 ± 0.8 | 4.3 ± 0.3 | 22.9 ± 2.2 | 12.6 ± 1.3 |
| RKO | WT | V600E | 5.2 ± 0.3 | 3.0 ± 0.2 | 24.5 ± 0.6 | 12.1 ± 0.8 |
| SW-1417 | WT | V600E | 4.4 ± 0.2 | 2.1 ± 0.2 | 24.2 ± 0.8 | 11.8 ± 0.9 |
A panel of human CRC cell lines with different KRAS and BRAF status was subjected to progressively lower concentrations of oxygen or glucose in the absence or presence of HGF, and the percentage of apoptotic cells was determined by flow cytometry using the annexin V/propidium iodide method. Values represent the mean ± SEM of 3 different measurements. CTR, control.