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. 2017 Mar 8;8(24):38239–38250. doi: 10.18632/oncotarget.16013

Figure 2. Selective targeting the growth of L1 ORF1/2 expressing cancer cells.

Figure 2

A. Immunoblots showing recombinant ORF1p expression in HEK293-T cells transfected with an L1 expression vector and endogenous ORF1 protein in HEK293-T cells, primary WI-38 and IMR-90 fibroblasts and the HT-29, MCF-7 and NCI-H1975 tumour cells. TUBA1A is used as a loading control. B. All indicated cell lines were seeded into a 6-well plate transfected with 4 μg per dish of the A5b or A1 vectors. Transfection was carried out using Lipofectamine 2000® (DNA/lipofectamine 1/3) for HT-29 and FuGENE® (DNA/FuGENE 1/6) for the other lines. After 48 hours, cells were re-transfected and 24 hours later treated with GCV (Sigma-Aldrich) at 10 μg/ml. Living cells were counted at the time of GCV addition (Day 0 =100%), and then at day 1 and day 2. All experiments were performed in triplicate and the data are expressed as mean ± s.d. with a pvalue determined by Student's t-test.