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. 2017 Mar 15;8(24):38294–38308. doi: 10.18632/oncotarget.16203

Figure 2. CD24+/high TNBC cells are resistant to docetaxel (DTX) and CD24−/low TNBC cells are resistant to doxorubicin (DXR).

Figure 2

(A) The drug sensitivity assay results of six TNBC cell lines dosed with either 25.6 μM docetaxel or 6.4 μM doxorubicin are shown. P-values were calculated with Two-way ANOVA. *P < 0.05; ***P < 0.001. (B) After seeding in matrigel for 4 days, cells were treated with 0.6 μM docetaxel or 60 nM doxorubicin for 7 days. (C) HCC1937 cells were cultured under standard conditions or in ultralow-attachment plates for 2 days, then treated with 6 μΜ doxorubicin or 25.6 μΜ docetaxel for either 2- or 7 days. ***P < 0.001; ****P < 0.0001. (D) Work flow for experiments shown in (E) and (F). HCC1806 and HCC38 cells were treated with 25.6 μM docetaxel for 2-days. Treated cells were then sorted into CD44+/CD24+/high and CD44+/CD24−/low populations by FACS. Sorted populations were re-treated with 25.6 μM docetaxel, with cell viabilities determined after 2-days. MDA-MB-468 and HCC1937 cells underwent the same protocol except 6.4 μM doxorubicin was used. (E) and (F) P-values were calculated with unpaired t test. *P < 0.05; **P < 0.01. Error bars represent SEM. Scale bar, 200 μm.