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. 2017 Mar 15;8(24):38294–38308. doi: 10.18632/oncotarget.16203

Figure 5. Bcl-2, TGF-βR1 and ATM signaling assessed by Western blot and FACS in selected TNBC cell lines.

Figure 5

(A) Cells were treated with 10 μM ATM inhibitor KU60019. (B) FACS results showed that 10 μM ATM inhibitor increased CD24 expression in MDA-MB-231 cells; and 4 μM doxorubicin reduced CD24 expression. (C) Selected cells were transfected with control vector, CD24 shRNA or NDRG2 shRNA. Knockdown of NDRG2 caused dramatic p-ATM increase in HCC1806 and MDA231 cells (D) The cells were treated with docetaxel 6.4 μM or doxorubicin 4 μM. Both docetaxel and doxorubicin increased p-TGF-βR1 in the three cell lines. Doxorubicin reduced p-Bcl-2 in HCC1937 and docetaxel elevated p-Bcl-2 in HCC1806 and HCC38.