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. 2017 Mar 16;8(24):38650–38667. doi: 10.18632/oncotarget.16246

Figure 3. BRET assay shows specific PAR2-LRP6 interaction in fibrocystic HU cells.

Figure 3

The existence of PAR2-LRP6 receptor-receptor interaction and SLIGKV agonist regulation is shown by quantitative BRET2 assay in fibrocystic HU cells. HU cells were co-transfected with Rluc-hPar2 and lrp6-pEGFP-C1. Specific maximal signal emission is detected beginning 5 min following SLIGKV activation (P=0.002) for up to 30 min, as compared with the non-activated status. This is a representative curve of three independent experiments performed.