Table 2. The primer sequences for quantitative RT- PCR used in this study.
Gene | Primer Sequence |
---|---|
mβ-actin | Forward: 5′-GAGACCTTCAACACCCCAGC-3′ Reverse: 5′-ATGTCACGCACGATTTCCC-3′ |
mTNF-α | Forward: 5′-CCCTCCAGAAAAGACACCATG-3′ Reverse: 5′-CACCCCGAAGTTCAGTAGACAG-3′ |
mIL-1β | Forward: 5′-TCGTGCTGTCGGACCCAT-3′ Reverse: 5′-GGCTTGTGCTCTGCTTGTGA-3′ |
mIL-6 | Forward: 5′-GGGACTGATGCTGGTGACAAC-3′ Reverse: 5′-CAACTCTTTTCTCATTTCCACGA-3′ |
mIL-8 | Forward: 5′-GGCATCTTCGTCCGTCCC-3′ Reverse: 5′-GCCAACAGTAGCCTTCACCC-3′ |
hGAPDH | Forward: 5′- ATCCCATCACCATCTTCCAGG-3′ Reverse: 5′- GATGACCCTTTTGGCTCCC-3′ |
hA20 | Forward: 5′- AATGCTAAGAAGTTTGGAATCAGG-3′ Reverse: 5′- CCGAGAACAATGGGGTATCTG-3′ |
hTNF-α | Forward: 5′- CACGCTCTTCTGCCTGCTG-3′ Reverse: 5′- GGCTTGTCACTCGGGGTTC-3′ |
hIL-1β | Forward: 5′-AAATGATGGCTTATTACAGTGGC-3′ Reverse: 5′-CTTGCTGTAGTGGTGGTCGG-3′ |
hIL-6 | Forward: 5′- CACTGGTCTTTTGGAGTTTGAGG-3′ Reverse: 5′- TGGGTCAGGGGTGGTTATTG-3′ |
hIL-8 | Forward: 5′- GACATACTCCAAACCTTTCCACC-3′ Reverse: 5′- AACTTCTCCACAACCCTCTGC-3′ |