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. 2017 Mar 28;8(24):39048–39063. doi: 10.18632/oncotarget.16587

Figure 2. CBX7 depresses the different tumorigenic degree of glioma cells.

Figure 2

(A-B) LN229 and T98G were treated with LV-CBX7 and performed colony formation assay. LN229/VEC, T98G/VEC, LN229/CBX7 and T98G/CBX7 stabled cells were counted and plated at a density of 400 cells per well and allowed for colony formation. Colonies were stained with crystal violet. Mean colony counts are displayed. Transfected cells exhibited a significant reduction of colony formation after 2 weeks. (C) CCK-8 assay displays decreased proliferation in LN229 and T98G after overexpressing CBX7. (D-E) The EDU showed repressed growth of LN229 and T98G after CBX7 overexpression. (F) The western blot indicates more of cleaved caspase-3 in LN229 and T98G after CBX7 overexpression, indicating more apoptosis. (G-J) After transfecting LV-CBX7, debilitated ability of migration and invasion was demonstrated by wound-healing assay and transwell assay. (K) Three-dimensional spheroid assays was carried out to display invasive ability after overexpressing CBX7. All experiments were performed in triplicate. * P≤0.05; ** P ≤ 0.01 and *** P ≤ 0.001.