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. 2017 Mar 28;8(24):39048–39063. doi: 10.18632/oncotarget.16587

Figure 4. Effect of CBX7 on the activity of the Wnt/β-catenin pathway.

Figure 4

(A) The effect of CBX7 on the Wnt signaling pathway was analyzed by immunoblotting with the indicated antibodies. (B) Difference of total β-catenin expression is presented statistically. (C) Cells were stained with an anti-β-catenin antibody (red) and DAPI (blue). (D) Cells were fractionated, and the nuclear and cytoplasmic extracts were subjected to immunoblotting. GAPDH and H3 were used as controls for the cytoplasmic and nuclear, respectively. The top three in Figure 4D correspond to LN229 and the bottom three correspond to T98G. (E) GSE4290, CGGA, TCGA database were used to analyzed the correlation between CBX7 and ZEB1. (F-G) ZEB1 expression in LN229/CBX7 and T98G/CBX7 stabled cells were subjected to immunoblotting. β-actin was used as an endogenous normalizer. * P<0.05; ** P < 0.01 and *** P < 0.001.