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. 2017 Mar 28;8(24):39048–39063. doi: 10.18632/oncotarget.16587

Figure 5. CBX7 enhanced DKK1 expression by binding promoter.

Figure 5

(A) CBX7 impact the expression of DKK1, analyzed by immunoblotting with the indicated antibodies. β-actin was used as control. (B-D) A luciferase reporter assay was performed to measure DKK-1 promoter activity in LN229 and T98G. TSS, transcription start site. (E) Schematic illustration of the promoter regions of the human DKK-1 gene and the regions containing the primers for ChIP assay (under). (F) The results of ChIP analysis show the CBX7 was recruited to the DKK-1 promoter regions in CBX7-overexpressing LN229 and T98G cells. IgG was used as an immunoprecipitation control. * P≤0.05; ** P ≤ 0.01 and *** P ≤ 0.001.