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. 2017 Mar 28;8(24):39048–39063. doi: 10.18632/oncotarget.16587

Figure 6. DKK-1 regulates the CBX7-mediated Wnt signaling pathway.

Figure 6

(A) Effect of the inhibition of DKK-1 on CBX7-induced increased activity of the E-Cadherin and oppositively on other invasion related proteins. Cells treated with DKK-1 siRNA were subjected to immunoblotting with appropriate antibodies. (B) CBX7-induced reduction of phosphorylated AKT and GSK3-β and ZEB1 were reversed by treatment of siDKK1. β-actin was used as an endogenous normalizer in immunoblotting. (C-D) Wound-healing assay, (E-F) Transwell assay and (G) three-dimensional spheroid assays were performed in LN229 and T98G treated with siDKK1. All experiments were performed in triplicate. * P≤0.05; ** P ≤ 0.01 and *** P ≤ 0.001.