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. 2017 Mar 27;8(24):39087–39100. doi: 10.18632/oncotarget.16598

Figure 1. CYP1B1 promotes cellular transformation of RWPE-1 cells.

Figure 1

(A and B) Endogenous expression of CYP1B1 protein (A) and mRNA (B) in PCa cells. Levels were determined by Western blot and qRT-PCR, respectively. (C–G) Effect of CYP1B1 overexpression on in vitro tumorigenicity. Ectopic expression of CYP1B1 in transfected RWPE-1 cells as examined by Western blot (C). Cell proliferation as determined by MTS assay (D). Colony formation as determined by crystal violet staining. Representative image of colonies (left panel) and quantification of stained colonies (right panel) are shown (E). Cell migration (F) and invasion (G) capability as determined by transwell migration and invasion assay, respectively. (H–L) Effect of DMBA treatment on in vitro tumorigenicity. Induction of CYP1B1 expression in RWPE-1 cells as determined by Western blot (H). Cell proliferation as determined by MTS assay (I). Colony formation as determined by crystal violet staining. Representative image of colonies (left panel) and quantification of stained colonies (right panel) are shown (J). Cell migration (K) and invasion (L) capability as determined by transwell migration and invasion assay, respectively. *p<0.05; **p<0.01.