Figure 5. PTEN lipid phophatase activity was decreased in HEK293 RONΔ165^E2 cells despite the upregulated PTEN protein expression.

(A) western blotting results using the antibodies against PTEN, P-PTEN(Ser380) indicated higher level in HEK293 RONΔ165^E2 cells compared to the HEK293 wtRON cells, p < 0.05. After co-immunoprecipitation with the antibody RON(C-20), western blotting with the antibody specific to PTEN showed that the RONΔ165^E2 protein had an obviously interaction with the PTEN protein in cytoplasm. 20% of total cell lysates were subjected to westernblotting with the PTEN antibody as input. (B) compared with the control group, in HEK293 RONΔ165^E2 cells it showed an increasing phosphorylation level of PTEN without the impact of MSP stimulation.