Figure 2. In vitro antiproliferative activity of ilaprazole.

(a, b) Expression of TOPK in lung cancer (A549), colon cancer (HCT-116), human ovarian cancer (ES-2) and pancreatic cancer cell (SW1990) lines. Tubulin was examined to serve as a loading control. (c) Graph indicates IC₅₀ values of ilaprazole in 4 TOPK-positive cancer cell lines. (d, e) The cell proliferation rate of HCT116 and ES-2 were remarkably decreased by ilaprazole. (f, g) Ilaprazole inhibits TOPK activity in HCT116 cells. The expression level of phosphorylated histone H3 (Ser10) and histone H3 was detected by western blot analysis. (h, i) Ilaprazole inhibits TOPK activity in ES-2 cells. The expression level of phosphorylated histone H3 (Ser10) and histone H3 was detected by western blot analysis. The relative intensity of p-Histone and Histone was calculated by an image J software.