Figure 3. Inhibition of Btk function in macrophages significantly compromises adhesion, invasion, and migration of lymphoid malignant cells.

(A) Lymphoid malignant cells were subjected to adhesion assays in conditioned medium collected from control and Btk inhibitor-treated (0.2, 0.5, 1, 2, 5 μM) macrophages. The adherent cells were measured by CellTiter-Glo luminescent cell viability assay. (B & C) Migration and invasion of tumor cells were analyzed in transwell plates, and supernatant from macrophages was added into the lower chamber as a chemoattractant. *Significantly decreased compared to LPS treatment alone (p < 0.05). Results are representative of three similar experiments.