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. 2017 Apr 11;8(24):39430–39442. doi: 10.18632/oncotarget.17049

Figure 5. Knockdown of Dvl3 and LGR5 suppresses stemness properties in HCC cells.

Figure 5

(A) Efficient knockdown of Dvl3 was established in Huh-7 cell line using sh#45 as compared to the non-target control (NTC). This suppressed sphere formation in vitro significantly and inhibited tumorigenicity in vivo. The median number of days of tumor onset was calculated based on the successfully formed tumors. (^ The numbers of days after the injection for the onset of the six tumors in the NTC group were 28, 46, 46, 60, 83 and 95, while those for the two tumors in the sh#45 group were 23 and 67.) (B) Efficient knockdown of LGR5 was established in Huh-7 cells using sh#86 as compared to the non-target control (NTC). This suppressed sphere formation in vitro significantly and inhibited tumorigenicity in vivo.