Figure 6. Selinexor combines synergistically with different classes of DNA damage agents.

HT-1080 cells were treated with selinexor and the FDA approved therapeutics in equimolar concentrations (A-D) Relative cell survival was detected with CellTiter-Glo after 72 hours (h). Combination indices for each combination were calculated using the median effect model. The dotted line represents a combination index of 1. Points less than 1 are synergistic whereas points greater than 1 are antagonistic. (E) A time course with selinexor or gemcitabine alone and in combination was performed at half the EC₅₀ concentration. Survival at each time point is determined via normalization to DMSO (mock) treated wells. A significant decrease in survival compared to mock treated is seen at 48 and 72h post treatment in the combination. Error bars are SEM from 3 experiments. ** is p<0.01 and * is p<0.05.