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. 2017 Apr 18;8(24):39605–39617. doi: 10.18632/oncotarget.17174

Figure 6. SphK2 enhances the K63-linked ubiquitin-dependent autophagic degradation of cytoplasmic RXRα.

Figure 6

(A) Western blots of LC3B, Beclin-1, p62/SQSTM1 and RXRα in HCT-116 and HCT-116Sphk2 xenografts in mice dosed with vehicle or ATRA. (B) Western blots of LC3B and RXRα expression in HCT-116 and HCT-116Sphk2 cells exposed to ATRA plus chloroquine (CQ) (10 μM) or 3-methyladenine (3MA) (1 μM) for 24 h. (C) Western blots of RXRα expression in HCT-116 and HCT-116Sphk2 cells exposed to MG132 (4 μM) to block the K48- based proteasomal degradation of cytoplasmic RXRα, in the presence of CQ (10 μM) or 3MA (1 μM) for 24 h.