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. Author manuscript; available in PMC: 2017 Jul 10.
Published in final edited form as: J Immunol. 2014 Jul 11;193(4):1911–1919. doi: 10.4049/jimmunol.1302392

Figure 1. Generation of TRIL-deficient mice.

Figure 1

A, Gene targeting strategy for generation of Tril-/- mice. The structures of WT allele, targeting vector and targeted allele are represented. B, PCR analysis of genomic DNA prepared from tail biopsies using primers TRIL-F1, TRIL-R1 and TRIL-R2, detecting both wild-type (179 bp) and mutant (415 bp) alleles. C, RT-PCR analysis of Tril expression in the brain of wild-type (+/+), heterozygous (+/-) and mutant (-/-) mice. Tril mRNA levels were normalized against Gapdh and expressed relative to the lowest detectable sample. Data are presented as the mean ± SD of one experiment representative of three independent experiments.