Figure 3.

Heterozygous tau knockout decreases the number of DA neurons in VTA at P0. A) TH immunohistochemistry of midbrains of P0 Mapt^+/+, Mapt^+/−, and Mapt^−/− mice. Representative coronal sections of midbrain from 4.83–5.07 mm rostral (12) are shown, with white dotted lines demarcating the boundary among the VTA, SN, and RRF. Scale bar, 200 μm. B, C) Number of TH⁺ neurons in total midbrains (B) and SN, VTA, and RRF (C) of P0 mice (n = 5 for each genotype vs. control littermates). Data are means ± sem. *P < 0.05.