Figure 2.

SRE.L activation upon U-46619 stimulation by Gα₁₃-YFP was comparable to wild-type Gα₁₃. A) Basal (white) and U-46619 induced (red) SRE.L activation by unlabeled and fluorophore-labeled Gα₁₃. Results are shown as relative (rel.) mean ± sem luciferase activity after normalization to nontransfected controls. Activation of the individual conditions by stimulation was tested with unpaired 2-tailed Student’s t tests (black lines and asterisks), and stimulation in the presence of the Gα₁₃ constructs was compared to stimulation in the absence of Gα₁₃ by ANOVA with Dunnet’s multiple comparison test (gray lines and asterisks). B) Expression of the different Gα₁₃ constructs [wild-type (wt) Gα₁₃≈44 kDa, GFP derivate≈28 kDa, top panel) during the SRE.L activation assay was confirmed by Western blot analysis. Actin (≈42 kDa, bottom panel) was used as the loading control. This experiment is representative of 3 individual experiments. ***P < 0.001, ****P < 0.0001.