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Sedimentation velocity analysis of mutant ALDH7A1 conducted at high concentration (~80 µM, monomer molecular weight). (A) Sedimentation velocity data for A129P (green), A149E (magenta), and G263E (purple). The result for wild-type ALDH7A1 (also ~80 µM) is shown for comparison (dashed black). (B) Sedimentation velocity data for G137V (red), G138V (blue), and G255D (orange). The result for wild-type ALDH7A1 is shown for comparison (dashed black).
