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. 2017 Jul 1;13:644–649. doi: 10.1016/j.dib.2017.06.048

Fig. 1.

Fig. 1

Immunodetection of phosphoinositide (3,4,5) triphosphate (PIP3) in liver cryosections. Immunostaining with a tetramethylrhodamine (TRITC)-conjugated antibody to detect PIP3 in mouse liver cryosections of each group (wc = wild type mouse standard diet; wm = wild type mouse MCD diet, kc = Lcn2-/- mouse standard diet and km = Lcn2-/- mouse MCD diet). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). As a negative control, non-specific antiserum was used for staining. Scale bars, 50 µm.