Subject area	Biology
More specific subject area	Liver biology
Type of data	Figures (Immunofluorescence staining, Western blot analysis)
How data was acquired	Microscope (Nikon ECLIPSE 80i) Chemiluminescent detection (Boehringer Mannheim GmbH Lumi-Imager)
Data format	Raw
Experimental factors	Liver sections were derived from WT and Lcn2-/-mice[2]after feeding a MCD or standard chow diet for 6 weeks. Protein extracts were prepared from the same livers for Western blot analysis. Hepatocytes were isolated from WT and Lcn2-/-mice for in vitro experimentation.
Experimental features	Liver sections from WT and Lcn2-/-mice were immunostained for PIP3. Negative and positive controls were also used. 100 µg of protein extracts from livers were used to detect PKB, phospho PKB, LCN2 and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) by Western blot. Hepatocytes were cultured from WT and Lcn2-/-mice. These were left untreated or treated with bovine insulin to induce PI3K and PIP3. Respective cells were immunostained for PIP3.
Data source location	Aachen, Germany
Data accessibility	Data not deposited elsewhere outside this article. Primary data are published in[1].