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. 2017 Jul 10;7:146. doi: 10.1186/s13568-017-0444-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Log₂ fold change of the target genes CYAN, amoA, and narG using 16S rDNA as a reference and compared to the t = 0 day community. R₂ values were >0.98 for all calibrations. qPCR reaction efficiencies were greater than 90%. Significant interaction effects were further determined with Tukey’s HSD test if the ANOVA results were significant (p < 0.05)—*p < 0.05, **p < 0.01, ***p < 0.001)