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. 2017 Jul 10;7:5009. doi: 10.1038/s41598-017-05400-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of C3 mutant piglets via SCNT. (a) Photograph of newborn cloned piglets carrying C3 gene mutation. (b) C3 genotypes of cloned piglets. The wild-type (WT) sequence is shown at the top in which the sgRNA sequences are highlighted in red, PAM sequences in blue, and insertions in green. WT: wild type; deletion (−); insertion (+).