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. 2017 Jul 10;7:5009. doi: 10.1038/s41598-017-05400-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C3 protein expression analysis of piglet serum. (a) Stain-free gel image of total loaded serum proteins. (b) Western blot of serum C3 protein of cloned piglets compared to that of wild type piglets. Full-length blot is presented in Supplementary Figure S3. (c) Serum C3 protein levels of C3 KO piglets and wild type controls were assessed by ELISA (**P < 0.01, data are shown as mean ± SEM, n = 3).