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. 2017 Jul 10;7:5009. doi: 10.1038/s41598-017-05400-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hemolytic complement activity of pig serum and effects on anti-human cells. (a) Total hemolytic complement activities were determined by Dian Diagnostics. (b) The percentages of 293T cell lysis mediated by various concentrations of WT pig serum, heat-inactivated WT serum, C3 KO piglet serum, and human serum. Lysis of 293T cells was significantly reduced when incubated in serum of C3 KO piglets compared to WT controls. (**P < 0.01, data are shown as mean ± SEM, n = 5). ND: Not detectable.