Table 3.

Details on the generated C3 KO piglets.

Piglets	Genotypes	Indel	Birth weight (kg)	Donor cell
	AG	wt
1#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.5	C-1
	GGCCGTCAAATGGCTGA--CTGGAG
2#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.85	C-1
	GGCCGTCAAATGGCTGA--CTGGAG
3#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.80	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
4#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	1.20	C-1
	GGCCGTCAAATGGCTGA--CTGGAG
5#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.95	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
6#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	1.05	C-1
	GGCCGTCAAATGGCTGA--CTGGAG
7#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.94	C-1
	GGCCGTCAAATGGCTGA--CTGGAG
8#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	1.08	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
9#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.705	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
10#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.804	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
11#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.979	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
12#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.938	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
13#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	1.067	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
14#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.970	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
15#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.605	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
16#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.450	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
17#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.360	C-1
	GGCCGTCAAATGGCTG--CCTGGAG
18#	GGCCGTCAAATGGCTGATCC----//--	+1, −198	0.577	C-12
19#	GGCCGTCAAATGG----TCCTGGAG	−4/−2	0.724	C-1
	GGCCGTCAAATGGCTG--CCTGGAG

sgRNA sequences are highlighted in red, PAM sequences in blue, and insertions in green. WT: wild type; deletion (−); insertion: (+).