Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 10;7:4942. doi: 10.1038/s41598-017-05232-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Longer mitochondria and round nuclei of microglia-derived macrophages in spinal cord tissues at the onset of experimental autoimmune encephalomyelitis. (a) After fixation and slicing (i), brain tissues were immunostained with DAB (ii), and embedded in resins (a, iii). Serial images were acquired for 3D reconstruction (a, iv). Reconstruction of nuclei (upper panels) and original immunoelectron microscopic images (middle, lower panels) of RFP- (b, red) and GFP- (c, green) positive cells in the spinal cord of a Ccr2 ^rfp/+::Cx3cr1 ^gfp/+ mouse with DAB deposits (b,c, asterisks). The nuclei of DAB negative cells (center) and immunocontrols (right) are also shown. The grading (d) and counting (e,f) of nuclear shapes in the RFP-positive monocyte-derived macrophages (MDM) and GFP-positive microglia-derived macrophages (MiDM). Single images (g,h) and nuclear (i,j) and mitochondrial (k,l) 3D reconstructions of MDM (g,k,i, red) and MiDM (h,j,l, green) are shown. Mitochondria are indicated (g,h, arrowheads). Long and short mitochondria are colored in blue and magenta, respectively (k,l). The mitochondrial length (m) and volume (n) of MiDM and MDM are compared. ns: not significant. ***p < 0.001 in the U-test. N = 648 or 786 for mitochondria in MDM or MiDM. Bars: 2 μm.