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. 2017 Jul 10;7:4942. doi: 10.1038/s41598-017-05232-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial elongation along microtubules following the stimulation with lipopolysaccharide (LPS). An experimental scheme shows that primary microglial cultures were quickly frozen (QF), freeze-substituted (FS), and immunostained for α-tubulin and the inner mitochondrial membrane (IMM) marker, COX I (a). Double immunostaining for mitochondria (COX I) and microtubules (α-tubulin) in microglia without the LPS stimulation (b, arrows) or 12 hr after the LPS stimulation (c, arrows). Areas indicated with rectangles (b3, c3, d3) are shown in the insets. Fluorescence intensity was measured along the lines (b3, c3, d3), and the arrows show the strong fluorescence intensities of α-tubulin (magenta) and COX I (green) at the same locations (b4, c4). Bars: 30 μm.