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. 2017 Jul 10;7:5008. doi: 10.1038/s41598-017-05264-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluxome experiment. (A) The main stages of the fluxome experiment include OMV incubation with isotope-labeled glucose with the following OMV isolation and purification. Extracted metabolites are utilized for metabolome analysis. (B) The reconstructed map of the ETBF OMV metabolic pathway (glycolysis). Labeled glucose metabolites such as ¹³C₄-D-erythrose-4P and ¹³C₃-Glycerol-3P were detected only in ETBF OMVs. ¹³C₆-D-Glucose and the products of ¹³C₆-D-Glucose catabolism, ¹³C₄-D-erythrose-4P and ¹³C₃-Glycerol-3P are indicated by red stars.