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. 2017 Jul 10;7:5006. doi: 10.1038/s41598-017-05231-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mode of transmission influenced the spirochete load of Borrelia afzelii in the Ixodes ricinus nymphs. Nymphs that acquired B. afzelii as larvae via systemic transmission had a mean spirochete load that was 26 times higher than nymphs that acquired the infection as larvae via co-feeding transmission. Shown are the medians (black line), the 25th and 75th percentiles (edges of the box), and the minimum and maximum values (whiskers).