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. 2017 Jul 10;7:4950. doi: 10.1038/s41598-017-05064-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of WAS on the expression of mast cell tryptase in the colon, ileal VE and FAE. (A–I) The tryptase⁺ cells increased in mucosal tissues of ileal VE, ileal FAE and colon in WAS rats, and decreased in cromolyn treated WAS rats. SED, subepithelial dome; LF, lymphoid follicles. Scale bars: 50 μm. (J,K) The expression of tryptase in colon, ileum VE and FAE were upregulated in WAS rats, and alleviated in WAS + Crom rats. N = 10 for each group. Data are displayed as the mean ± SD. *P < 0.05, **P < 0.01, compared with SS group; ^#P < 0.05, compared with WAS group. followed by IBS-D FSN stimulation (**P < 0.01). The gels were run under the same experimental conditions. Cropped blots are displayed and full-length blots are included in the supplementary information.