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. 2017 Jul 10;7:4928. doi: 10.1038/s41598-017-05401-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LepR⁺ cells in the bone marrow cavity express Runx2. (A–H) Z-stack confocal (A,D–F and H) and confocal (B,C and G) images of thick bone sections of Runx2-GFP mice (5–6 weeks old). The images were taken from whole bone tissue (A), endosteum (B,C and F), epiphyseal cartilage (D) and bone marrow (E,G and H). Bone tissues are stained with osteocalcin (Red) (B), SOST (Red) (C), Type-2 collagen (Col2) (Red) (D), CD45 and Ter119 (Red) (E), VE-cadherin (VE-Cad) and CD31 (Red) (F), Perilipin (White) (G), and Leptin receptor (LepR) (Red) (H) antibodies. Nuclei are visualized with Hoechst 33342 (blue) and propidium iodide (PI) (Red). Arrows: Osteoblasts (B), Osteocytes (C), and LepR⁺ cells (H).